FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3061699
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11650.040.1937451
TCTGTCG11300.038.8670168
TCGCTCT12100.034.512184
CGCTCTG13900.031.5969225
GTCTTGC23600.031.4409921
CTCGCTC14500.029.7929173
TTCACCG8250.029.672545
TACACCG2452.1263986E-929.3875715
TCTCGCT14300.028.6991212
GTGTTAG17850.027.0400541
GTATTAG8700.026.4973051
GTATTAA6750.025.6140631
ACCGTGT9600.025.499848
GTCTTAC9500.025.0242671
TCTGTCA30500.024.5506678
GTACTAG5000.024.4934461
TCACCGT11500.024.417246
CCGTGTT9250.024.1295789
GTATAAT11500.023.8043731
CTGTCGC20750.023.5950349
Produced by FastQC (version 0.11.5)