FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2311539
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8800.039.7687151
TCTGTCG9850.038.734798
GTCTTGC16650.037.220971
TCGCTCT10200.034.582594
GTGTAAC5750.034.2356761
GTATAGG5950.030.6341631
GTCTCAC20000.030.2573621
TCTCGCT11000.030.1328542
GTTACGC1000.006290022328.7952963
CTCGCTC10950.028.2693563
GTGTTAC6900.027.4730721
TTGCTCT21300.027.037844
GTATTAG6500.026.920361
GTCTTAC6600.026.5124761
GGGTTTC29100.026.3074841
CGCTCTG11500.026.2913595
TCTGTCA23050.026.2343278
GTCCTAC6500.025.798681
CTGTCGC16050.025.5659189
GTCTCGA18400.024.6481486
Produced by FastQC (version 0.11.5)