FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2155490
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7850.039.4393581
TCTGTCG8500.036.4242488
GTCTTGC17850.033.8822361
TCGCTCT9450.033.5236974
TCTCGCT10150.029.7930032
TATTACG1000.006285018328.7999022
CTCGCTC10950.026.3012813
GTGTTAC7150.026.181731
GGTATAC4950.026.181731
TTGCTCT23650.025.5728534
GTGTTAG15600.024.4614561
GTATTAA5300.024.4527471
GTGTTAA7700.024.3116051
CTCGATC8100.024.0004758
GTCTCGA19200.023.9999186
TCTCGAT8300.023.4221527
GTATTAT5850.023.3845351
CTGTTGC20650.023.3612389
TCGATCT7750.023.2262659
GTACTAG4053.45608E-1123.1110321
Produced by FastQC (version 0.11.5)