FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2112280
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG6650.037.926561
GTCTTGC15300.031.5558871
TCTGTCG9300.030.968078
GTCTCGC9350.030.0573021
GTTACGC1000.00628456428.80033
GTCTTAC7600.028.4449181
GGTATAC4150.027.7823471
TCGCTCT10650.026.3664744
GTGTTAG14800.026.292331
GTGTAAC6600.026.2038021
GTTAGCC10200.025.4120313
GTATAAC3751.2732926E-1124.9809591
GTCTAGG10200.024.0201531
TACACCG2401.698616E-624.0002525
GTCCGTA1550.001874292323.2453121
TCTCGCT12200.023.0166362
GTGTTAC7900.022.8039461
ACTTTCG1902.2993924E-422.737088
GTCTCAC19200.022.5188921
TTGCTCT22800.022.4212884
Produced by FastQC (version 0.11.5)