FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2104009
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC16600.033.3973581
GTATTAA5050.032.7918551
GTCTCGC7350.030.3671361
GTCTAAC3350.030.0893441
GTATTAC4100.029.853451
TCGCTCT8250.029.6725224
TCTGTCG6100.028.3276718
GTCTTAC7450.028.0266511
TTACACT7950.027.1696224
GTATAAC3451.1823431E-1025.0433061
GTACTGA5200.024.9229051
TTGCTCT21250.024.7339464
TCTCGCT9050.024.6628132
GTATTGA5900.024.4066121
TATTACG1500.001556348923.9998322
GTCTAGC5850.023.3844531
GTATTAT4750.022.7366851
GTATTGC5400.022.666511
CGCTCTG8900.022.651535
GTTTCAC21700.022.5620563
Produced by FastQC (version 0.11.5)