FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091412
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT8900.033.1683464
TCTCGCT8700.032.2756732
TCTGTCG7850.031.1845328
GTCTTGC16950.031.0086691
GTCTCGC8550.030.3156131
GTACTAC3151.8189894E-1229.7141111
TACACTG10550.029.3458025
TCTGTCA21050.027.0212218
TTGCTCT22000.026.5089384
GGGTTTC26150.026.4319691
CGCTCTG10900.026.4218655
TTACACT7600.025.57884
GTGTAAG9350.024.6415671
CTCGCTC11650.024.1028653
GTGTTAC7500.023.999861
CTACGCT2401.6988433E-623.999864
GTGTAAC5800.023.586071
TGTTAGC9850.023.3907262
CTGTCAC25000.023.3278669
TATACTG5250.023.314155
Produced by FastQC (version 0.11.5)