FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2091412
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7850.038.541241
TCTGTCG8000.037.800518
TCGCTCT8500.035.5760964
GTCTTGC16450.033.7187041
CGCTCTG10100.029.2274175
GTACAGG10400.027.7059271
TCGTTCG1050.00797462327.428948
TATACTT5000.027.3597135
TCTGTCA19300.026.4874048
CTCGCTC11150.026.475063
TCTCGCT11700.025.2305072
TTGCTCT19550.025.0432174
CAATACT4350.024.8273264
TAGTACT3551.6916601E-1024.3377744
GTACTAT3902.1827873E-1124.0118051
GTGTAAC6650.023.8312631
CTGTCAC24200.023.8019739
GTCTCGA14600.023.6709866
GATCGTT2202.3526307E-522.9093999
TCTTGCT21200.022.7544782
Produced by FastQC (version 0.11.5)