FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051419
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7750.041.7947048
GTCTCGC8600.038.8253941
GTCTTGC15700.034.675131
TCGCTCT8900.033.967984
GTATTAC3650.033.807461
TCTCGCT9650.032.819792
GTATAAC4100.030.0968841
GTATAGC3950.029.402171
TTCACCG4500.028.7919085
TTACACG2354.6136847E-827.566724
GTGTACG2654.5056368E-927.3911721
GTATTAG5650.026.9790941
CGCTCTG10750.026.783175
TCATACT6450.026.783174
GTATAAG5450.026.6372851
CTCGCTC10850.025.8729133
GTGTATA8000.025.4053121
CTGTCGC13950.025.2832159
TATACTG5550.024.6417245
GTACTAA2951.4117177E-824.605631
Produced by FastQC (version 0.11.5)