FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2506933
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8750.042.778558
TCGCTCT11850.037.6620674
GTCTCGC11250.036.1175961
GTCTTGC19150.033.721321
CTCGCTC13650.032.6949843
TCTCGCT13000.032.6686172
TAATACT5200.031.8386964
GTGTTAG14750.027.5473181
CGCTCTG15000.027.353595
GTTTCAC28750.027.0400813
GGGTTTC32200.026.8147891
GTCTTAC7600.026.7317731
GTACTAG5250.026.2589661
AATGGCG12900.025.6684065
CTGTCGC15750.025.5940069
GTTAGCC11000.025.5207843
GTGTATA10900.025.2953341
GTGTTAT8750.024.0476861
GTCTCAC22500.023.8634131
GGTTTCA30900.023.2950482
Produced by FastQC (version 0.11.5)