FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175213
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9600.043.0440861
TCGCTCT9950.040.510634
TCTGTCG7800.039.6805738
TCTCGCT10500.036.3321152
GTCTTGC17500.036.0405241
CTCGCTC11100.034.3682173
GTATTAT4700.030.8490641
TAATACT4500.030.3910124
GGGTTTC26600.029.1616441
GTACTAA4000.028.9981211
CGCTCTG12700.028.9048395
GTCTCAC20400.028.78491
GTATTAG5900.028.260881
GTTCTAC4650.028.0626961
CTTGCTC21900.027.2796043
GTTTCAC26200.027.1980633
TTGCTCT23300.026.567254
TTGTACT6500.025.469394
GTATTGC5700.025.4369471
CTGTTGC19750.025.1469949
Produced by FastQC (version 0.11.5)