FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345160

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345160
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175213
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG553.3350766E-452.3530465
TCTGTCG7150.049.3326768
GTCTTGC18450.035.551251
CGTCGTT900.003757966731.9935287
TTGCTCT22100.030.9439684
CTGTCGC12800.029.2440859
GTACGGC1504.2468615E-529.1520251
GTGTAAC5350.028.6071261
TCGCTCT7950.028.0697944
GTCTCGC8050.028.0656131
ACCGTGT6450.026.785288
GTCTCAC19900.026.7348961
TACACTG11050.026.709535
GTTTCAC24900.026.020033
GGTATAC4550.025.6281531
GTATTAT4900.025.284921
CTTGCTC22600.025.1642073
TTACACT8650.024.9660474
GGGTAAT5600.024.7271651
GTATATA6550.024.4788021
Produced by FastQC (version 0.11.5)