FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345161

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345161
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1137675
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4550.052.2157148
GTCTCGC4550.041.141461
TCGCTCT4700.038.294894
GGGTCGA1001.4500441E-435.99726
CGCTCTG4950.034.9063725
GTCTTAC4000.034.198841
CTGTCGC6650.033.56139
CTCGCTC5100.031.0564043
TCTCGCT5400.030.6656282
GGATAAT4250.030.4930861
TAATACT3151.8189894E-1229.7119754
GTCTTGC8000.029.6989961
GTGTACG1703.1799118E-629.6460551
GTACTGC3201.8189894E-1229.249011
GGGTAAC3450.029.2164021
GTACTAT2752.3101165E-1028.7990251
GTGTAAC2853.5106495E-1027.7885321
GTCTCAC12700.027.7785851
TCGATCT4000.026.9978989
GGTAACC2456.8890586E-826.4480842
Produced by FastQC (version 0.11.5)