FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365766
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7650.048.9316258
GTCTTGC19300.036.8436781
GTGTTAG12500.033.0871241
GTCTCGC9900.032.2486651
GGTCCGA1902.0637162E-730.5513631
TGTACCG1204.2325066E-429.9941445
GTATAAT5650.029.537491
GTATAAG5150.028.1784421
CTGTCGC15050.027.7420929
TAATACT5750.027.5424484
TCGCTCT11300.027.3928814
TTGCTCT24750.027.3401174
CGGTACT1358.429112E-426.6614634
GGGTTTC31350.026.6167181
CCGTTAT1100.00963559126.3852671
TCTCGCT11550.026.1767062
GTTTCAC28250.025.9913853
TATACTG6950.025.8942245
GTATTAC3657.2759576E-1225.8431031
TTATACT6700.025.7860094
Produced by FastQC (version 0.11.5)