FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2365766
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9100.053.6821
TCGCTCT8900.045.296974
TCTGTCG8300.045.102068
CTCGCTC11350.037.4220243
TCTCGCT11700.036.337122
GTCTTGC17950.034.9324651
GTATTAG6750.032.4050521
GGTATAC4350.031.8463421
CGCTCTG11950.031.3261155
TAACACT9450.029.7100854
GTATTAC5250.029.1645451
GTCCTAT5000.027.706321
GTCTTAC7700.027.4601251
GTATTAT6400.027.3417631
AATGGCG12450.027.176885
ATGGCGT11000.026.832576
GTGTTAG14900.025.9349171
GCGTTTA1709.9294615E-525.7334231
GGGTTAT4850.025.5565621
CTGTCGC15700.024.760839
Produced by FastQC (version 0.11.5)