FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345167

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345167
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206503
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC17950.037.1859471
TCTGTCG7100.036.4981778
GTATTAA5450.035.9436951
GTATAAT5600.032.3897361
GTCTCGC7950.031.9415761
TCGCTCT8600.028.4582044
GTTAGCC9650.026.8535823
GTACTAC3357.4578566E-1125.989141
TTGCTCT21900.025.6376084
GTATTAC3709.094947E-1225.49161
GTGTTAC7500.025.151711
GTGTTAG13900.025.0542771
GTTCTAC6450.024.7467631
GAGTGCA43200.023.6609216
GTCCTAC7400.023.5307081
TGTACGC1851.92275E-423.345682
GGAGTGC40150.023.3069175
TGTTAGC11500.023.1595942
CTTGCTC23200.022.959943
GTATAAA7300.022.8591671
Produced by FastQC (version 0.11.5)