FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206503
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7950.037.6019251
TCTGTCG8900.036.3981638
GTCTTGC18400.030.9079461
GTATTAG5950.028.1841031
TACCGTC1306.760093E-427.687497
GTTTCAC25700.027.4511013
TCGCTCT9250.027.2385064
TAATACT4600.026.6040694
GTGTTAG12500.026.247981
GTCCTAT4450.026.2152081
GGGTTTC29500.026.1985471
GTGTTAC7250.026.1474111
TCTCGCT9950.026.0705322
GTCTTAG7850.026.006491
GTACAAC5050.025.98811
GTATTAA5350.025.8936461
CGCTCTG10400.025.610935
TATACTG5650.025.4822945
GTCTATA3451.0186341E-1025.3603691
ACCATGT21900.025.3106678
Produced by FastQC (version 0.11.5)