FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2709371
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10450.034.7222441
GTCTTGC22650.033.6414831
TCTGTCG9350.031.565288
GTGTTAC9600.029.4813541
GTCTTAC8400.027.645521
TCGCTCT13000.027.132524
TTGCTCT29650.026.948574
GTTACCC8700.026.4769613
TCTCGCT14000.025.7086562
GCTATAC4300.025.110783
GTACTGA6750.024.7273831
GTTTCAC29950.024.2751523
CTTGCTC28550.023.700593
TCGATCT10100.023.5196049
GTATTAG6800.023.4783631
TACACTG12950.023.346245
GTGTAAC7250.023.0220471
CTACGCT2202.3571929E-522.9040764
CGCTCTG14150.022.8925135
GTATTAC4455.456968E-1222.830851
Produced by FastQC (version 0.11.5)