FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533566
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5100.043.76628
GTCTTGC12600.034.2846531
TCGCTCT6400.033.7511564
GTCTCGC7200.032.9989781
TCTCGCT6850.031.531872
CGCTCTG7350.030.3683855
CTCGCTC7950.029.8878143
CTGTCGC8300.028.6274859
GGGTTTC18450.026.9259951
AGCACCG3505.456968E-1226.743775
GTGCTAA4450.025.8868391
GTTTCAC16650.025.5143873
GTTAGCC5000.024.4808373
TTGCTCT16300.023.8535794
GTCTTAC5500.023.5629061
TAGGCCG2452.0312546E-623.511015
GGTAACG1851.9196204E-423.3506282
GTATAAC3402.7175702E-923.2933961
TCTTGCT17050.023.2250862
ACCATGT14650.023.0997668
Produced by FastQC (version 0.11.5)