FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1533566
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC11700.036.328351
GTCTCGC6600.031.654361
TCTGTCG5750.031.3037768
GTATTAC3050.030.7059821
ACCGTCG950.0048912830.3152338
GTGTTAC5350.029.6243211
TCGCTCT6450.029.0227244
GTACTAA2752.2919266E-1028.8163851
GGGTTTC18800.027.973351
GTGTTAG8300.027.774831
GGTACCG1306.7530014E-427.6918015
GTATTGG4000.027.015361
TAGCGCA1358.419054E-426.6661787
GTTTCAC17450.025.9980323
GGGTAAG5050.025.6779671
GTCTCAC13050.025.393751
GTCTAAC3151.1132215E-925.157161
GAGCCGT3751.2732926E-1124.9595436
GTATTGA3801.4551915E-1124.645591
GGATAGG5600.024.4424691
Produced by FastQC (version 0.11.5)