FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244298
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG4300.046.8811728
GTCTTGC10200.040.93731
TCGCTCT4600.040.693444
GTCTCGC4550.037.9744261
GTACGGA1157.4963737E-637.5616651
TTGCTCT13550.036.1308754
CTTGCTC13300.034.1034053
TAATACT2757.2759576E-1231.416474
CTGTCGC6300.030.8554639
CCGCGCG2651.5097612E-1029.8851649
TCTTGCT13200.029.451762
TCTCGCT5500.028.7972742
CGCTCTG5850.028.3061545
GTTCTAC3850.028.0492941
CAATACG1050.00797604327.4270784
TTACACT5000.027.358514
ATACTGA5300.027.1683316
CTGAACG1358.419931E-426.6652159
GTCTCAC11450.026.407981
TACACTG6900.026.0855375
Produced by FastQC (version 0.11.5)