FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1244298
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC4900.048.500341
TCGCTCT5450.047.5585024
CTACGGT700.001093464441.141884
CTCGCTC6300.037.714913
TCTCGCT6500.037.662162
CGCTCTG6700.037.611055
GTCTTGC9600.036.0078281
CGACCGT800.002107420535.999156
TCTGTCG4800.035.9991468
GTCGTGC1501.1974462E-633.59928
GTACTAC2009.558789E-932.4070471
GTATTAG3400.031.7716141
GTGTAGC5350.030.9600031
GGTATAC2809.094947E-1230.8638531
CCCGTCG1402.8806662E-530.856418
ATGGCGT5500.030.108386
GTACTAT2701.8553692E-1029.3397121
GTCTCAC12000.028.8062631
CCTTACG1000.006284636428.7993168
GTCTTAG4150.027.7650721
Produced by FastQC (version 0.11.5)