FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649370
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10100.041.3441358
GTCTCGC12050.036.448081
GTCTTGC21400.036.33641
GTATTAA6200.035.999951
CCGTTAG1201.0034837E-535.9999471
TCGCTCT11400.031.5771144
GTACCGA1402.8814453E-530.8570981
TAATACT6550.028.5784954
TCTCGCT13900.028.4875552
CTGTCGC15450.028.4260719
GTATAGA8300.026.024061
CTCGCTC13900.025.8977783
TTACACT11200.025.7127914
GTCTCAC24650.025.7038171
GTACTAA5100.025.4117261
GGGTTTC32900.025.3859831
CGCTCTG12600.025.1413975
TTGCTCT27300.024.5260494
GTATTAG8350.024.1436791
CTTGCTC26900.024.0878223
Produced by FastQC (version 0.11.5)