FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2649370
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11250.039.6782578
GTCTCGC9000.038.4447481
GTCTTGC20600.035.692031
TCGCTCT10550.034.1217274
TTGCTCT24800.029.3212954
CTCGCTC12350.029.1523693
GTATTAT5750.028.8335631
GTATTAA5550.028.5738011
GTGTTAG15650.028.5572011
CTTGCTC25300.027.8917873
GGGTTTC34300.027.7407461
TCTCGCT12900.027.364682
GTTTCAC30550.027.10553
CTGTCGC16450.026.2602469
TCTGTCA25050.025.8671288
GTTAGCC12400.024.9699443
TGTTAGC14450.024.9279252
GTGTTAC8450.024.7388551
CTATGCG2051.3629093E-524.5842869
TCTTGCT30750.024.3653262
Produced by FastQC (version 0.11.5)