FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345181

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345181
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences886692
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCCGT700.00109372941.1385576
GTCTCGC3650.035.505141
GTCTAGT2053.1468517E-1035.120261
CCGTCGG1102.5376352E-432.7256979
GTCCTAT2009.576979E-932.3984381
TCGCTCT3800.032.2071574
TGCATCG1153.2947815E-431.3010775
GTATTAT2351.3606041E-930.636821
TCTCGCT4050.030.219062
TACCTCG1204.2281498E-429.996865
TCTGTCG3151.8189894E-1229.711188
GTCTTGC7300.029.5876161
TTATACT2452.1245796E-929.3846824
GTCTAAC1000.006284427428.798611
TATTAAC2003.4599543E-728.7969862
ATACTGA4300.028.462146
CTACACT4050.028.441474
GTCTCAC7950.028.0741211
CGCTCTG3600.027.9970725
GTACTAT1555.7671357E-527.8696251
Produced by FastQC (version 0.11.5)