FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2882130
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9600.047.2475178
GTCTCGC11700.043.077651
TCGCTCT12700.037.415364
GTATTAG7250.036.7454451
GTATAAC5000.034.560581
GTCTTGC22500.034.2405781
CTCGCTC14700.031.8350643
TCTCGCT14000.031.3697812
GTGTTAC9250.030.3572671
GTATTAC5500.030.1095981
GTCTTAC9900.029.0913981
CGCTCTG15450.028.4256925
CTGTCGC17150.028.1272899
GTATAAT6600.026.182261
TAATACT6050.026.180444
TTGCTCT27800.026.1568984
TAGACCG2508.389361E-825.9186385
CTTGCTC29400.025.7129363
ACCGTGT7900.025.5176458
GTACTAC4900.024.9800131
Produced by FastQC (version 0.11.5)