FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641000
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9100.038.7670678
GTCTTGC20450.033.451
GTCTCGC10900.032.3694571
GTGTTAG14950.031.7883951
TCGCTCT10300.030.7555664
GTATTAA6750.028.8022121
GTCTTAC7950.028.0776271
GTTAGCC12900.026.2310963
TCTCGCT12150.026.0726222
TTGCTCT28250.025.9950124
CTGTCGC15100.025.271079
GTATAAA6850.025.2282141
CTCGCTC12950.025.0179123
CTGTTGC23700.024.9109469
GTATAGA6950.024.865221
CGCTCTG12700.024.3765955
GTGTTAA8400.024.0018421
GTATAAT5750.023.793131
GTATTAG6150.023.4164331
TGTTAGC15100.023.3629362
Produced by FastQC (version 0.11.5)