FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2641000
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11000.042.5441478
GTCTCGC9650.036.586861
TCGCTCT11700.035.691214
GTCTTGC18900.033.9300581
TCTCGCT12300.032.2093772
CTCGCTC13300.030.8591143
CTGTCGC17050.027.8701119
GTCTTAC8300.027.7797451
GTACGCC1607.1587456E-527.0017263
CGCTCTG14600.026.6293185
GTATAAC4350.026.5025141
GTCTCAC25300.026.2013511
TACCCCG2508.387178E-825.9192035
GTACTAG3801.4551915E-1124.6499541
TTGCTCT23900.024.4009234
GGGTTTC30000.024.0179061
TTATACT5400.023.9992624
GTGTTAG14450.023.9347971
GTCTAAA7050.023.5068841
CTTGCTC24850.023.4703123
Produced by FastQC (version 0.11.5)