FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2945375
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10500.047.998548
GTCTCGC12050.040.667441
GTCTTGC21850.039.5780831
TCGCTCT12250.038.7906574
GTCTTAC8450.033.260811
TTGCTCT27800.031.5961634
GGCTAGA15300.031.5579191
CGCTCTG14250.031.3253635
TGTACCG1902.20949E-730.3148675
GTTTCAC31600.029.8512483
GTGTAAC6950.029.033421
GGGTTTC35250.028.8260381
TCTCGCT16050.028.725012
CTGTCGC18600.028.6442899
GTATTAT6550.028.6059931
GTCTCAC26900.027.32581
CTCGCTC16100.027.282383
CTAGAGT12500.027.0748523
CTTGCTC29100.026.9719143
TAATACT6150.025.7553144
Produced by FastQC (version 0.11.5)