FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3004842
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG11050.045.608028
GTCTTGC22500.036.4807661
GTCTCGC13250.032.604461
TCGCTCT12650.030.7332694
GTGTTAG18350.029.0360341
TCTCGCT15200.028.4192832
TTATACT5950.027.8302024
GTCTCAC30550.027.5750481
CTGTCGC19400.027.0920039
GTATTAC5150.026.5636631
CTCGCTC14950.026.4866473
GTTTCAC38450.025.2780133
GGGTTTC41600.025.269761
TTGCTCT29700.025.2105564
GTATTAG7150.025.1753561
CTTGCTC30900.025.1634853
GTGTTAC10600.025.1326031
GTATTAA6600.025.0914361
GTATAAT5750.025.0440041
TCTTGCT34250.023.5430622
Produced by FastQC (version 0.11.5)