FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2480316
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8550.038.7346888
GTCTCGC10750.034.8540761
GTCTTGC19950.033.9503021
TCGCTCT10950.033.5323834
ACCGCGT2009.59335E-932.39828
GTGTTAC8550.028.6530911
TCTCGCT12900.027.3605562
CTCGCTC13450.027.302353
CGCTCTG12350.026.2333585
GTATAAC4950.026.201491
GGGTTTC31350.025.7418141
ATACCGT2301.1734282E-625.0420886
CTGTCGC15100.024.7933229
TTGCTCT26800.024.7150444
GTGTTAG14300.024.1859911
GTTTCAC28250.023.9586073
TCTGTCA25600.023.6236868
GGAGTGC52250.023.4245345
GGGTTAC7100.023.3414691
GTCTCAC23250.023.2432581
Produced by FastQC (version 0.11.5)