FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136447
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7900.041.9228068
TCGCTCT8600.040.184864
GTCTCGC9300.037.16281
CGCTCTG10500.033.5990075
GTATTAT5150.033.5547641
TCTCGCT10000.032.399042
GTCTTAC7200.031.001261
GTCTTGC15350.030.9589121
GTGTAAC5850.030.7704811
CGTTTCG1453.6618738E-529.7922219
GTATTAA5100.028.2364431
CTCGCTC11500.028.173083
TCTCGAT8400.027.427767
ACCGTGT6600.027.271928
GTCTCAC21750.025.8217371
GTCTCGA18750.025.343256
GTTAGCC9000.024.7992673
CTGTCGC13650.024.7904769
TTGCTCT21300.024.3373094
GTACCGT1500.001555925724.0009751
Produced by FastQC (version 0.11.5)