FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136447
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7600.040.7354978
GTATTAG6650.040.103881
GTCTCGC7650.038.630361
TCGCTCT8550.035.3672524
GTCTTGC17300.034.5810621
TCTCGCT9350.032.3563232
TTCACCG6050.029.7510835
CCGTGTT6500.028.7990519
ACCGTGT7500.028.799058
AATAGCG2253.0173396E-828.799055
CTCGCTC9550.028.649613
GTCTTAC7050.028.626961
GGGTTTC27250.028.3024231
CTGTCGC13050.028.1370039
TACACTG10250.027.3942175
GTTTCAC24900.027.1810993
GTATAAC3453.6379788E-1227.1600511
GTGTTAG12200.026.5863671
TCTATCG1658.830852E-526.1809548
CGCTCTG10100.025.662525
Produced by FastQC (version 0.11.5)