FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2248605
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8400.038.567718
GTCTTGC19050.034.3903851
GTCTCGC8950.030.5668891
GTATAGA6900.030.2579541
GTGTTAG12750.029.9265291
TCGCTCT7700.029.9191934
GTATTAA6100.027.1449241
GTGTAAG10650.026.3636571
CTGTCGC13500.026.1308169
GTATTAC4700.026.0400451
GTGTTAC7500.025.9175031
TTGCTCT25200.025.7118074
GTGTAAA11250.024.9575941
GTAATAC4650.024.7718073
TATACTG4950.024.7248925
GTGTATA9400.024.5082761
GTGTTAT7650.024.468231
GTATAAA6250.024.1896691
TCTCGCT9850.024.1195032
TAATACT5450.023.7775254
Produced by FastQC (version 0.11.5)