FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2371941
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9800.041.8783041
GTCTTGC19100.039.5818631
TCTGTCG10650.033.8027158
TCGCTCT10600.032.604364
CTCGCTC11450.030.1839493
TTAGACT5350.028.262194
GTATTAG5700.027.7899741
TCTCGCT12250.027.6249892
TTGCTCT23850.026.5665154
GTTTCAC25550.026.2079093
CGCTCTG12400.026.1295035
GGGTTTC28400.026.1131461
GTGTTAG13950.024.774641
GCGTTAC1751.316713E-424.686161
TCTTGCT27300.024.5279142
CTTGCTC23200.024.5176833
GAGTGCA51550.024.3030556
GGGTAAT6000.024.0004311
GTATTAA4850.023.7530061
GGAGTGC48800.023.6069835
Produced by FastQC (version 0.11.5)