FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345202

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345202
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2662899
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11950.040.390721
TCTGTCG12000.040.1975028
GTCTTGC20850.036.9702531
TCGCTCT12750.036.7049834
TCTCGCT15350.030.9568982
GTCTTAC7500.030.7371391
GTATTAC4750.030.3327051
CTCGCTC15900.029.4332433
TATACTT5800.028.5499535
GTGTAAT9200.027.4065861
TTGCTCT27850.026.8862344
CTCGATC10450.026.8691468
TTATACT6200.026.709024
TACCCCG2757.2996045E-926.1801955
CGCTCTG14600.026.1353655
GTGTTAG15000.025.9344621
GGGTTTC35050.024.8697871
GTTAGCC12550.024.6687163
GTTTCAC32650.024.4771943
GTATTAT6550.024.1966971
Produced by FastQC (version 0.11.5)