FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195028
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT9350.041.5837024
TCTGTCG9200.041.478138
GTCTCGC10100.039.9270321
GTCTTGC15350.036.122911
CTCGCTC10700.033.645523
CGCTCTG10550.033.4414145
GTGTTAG12650.031.87851
GTCTTAC6000.030.0046881
CTGTCGC14650.028.5050329
GTATTAC4600.028.1783161
TCTCGCT12850.028.0161132
GTACCGT1306.752439E-427.692856
GTCTCAC19300.027.6105331
TAGTACT4000.027.0005284
TTGCTCT20950.026.4635264
GTATACG1400.00104008925.7183061
GGGTTTC28400.025.3560751
GTTAGCC10150.024.8280743
GTTTCAC25600.024.7504863
CGCTAAG1751.3166547E-424.68627
Produced by FastQC (version 0.11.5)