FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1771642
Sequences flagged as poor quality0
Sequence length150
%GC52

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7200.044.0036961
GCGTTAC1105.519747E-639.276031
GTCTTAC5300.036.682331
TCTGTCG7300.036.493138
GTGTTAG9550.036.1915251
CGTTACC1201.0032907E-535.9999772
GTTAGCC8700.035.586193
GTCTTGC12750.035.579461
GTACGGA1708.313327E-833.88521
TGTTAGC9050.033.4143452
TCGCTCT8650.033.294784
TCTCGCT9900.032.7272532
CTCGCTC9350.031.5721743
CGCTCTG9050.031.0276055
GTATTAC3151.8189894E-1229.7167841
ATGGCGT7300.029.5890256
TTACACT6100.028.3278524
GTCTCAC17650.027.741711
GCGTAAT1050.007971548527.430881
GTATTGT4600.026.6109311
Produced by FastQC (version 0.11.5)