FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1706152
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC11900.038.7259671
TCTGTCG6950.037.2948468
GTCTCGC6900.034.43771
GTATTAG4550.033.233581
GTATTAA3500.032.9170721
GTACTAT3750.032.642761
TAATACT3700.031.1359444
GTCTCAC15400.028.5218981
TCGCTCT7650.028.2360294
GTGTTAG9750.028.0639151
CTGTCGC11050.028.018019
TTGCTCT15900.026.7176784
GTACTAA2508.3740815E-825.9221951
TTATACT4450.025.8883154
GGGTTTC22100.025.7397371
CTCGCTC8700.025.655843
CGCTCTG8200.025.4640775
TATACTG5100.025.4124265
GTTAGCC7750.025.0845243
GAGTGCA35050.024.856576
Produced by FastQC (version 0.11.5)