FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358741
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9050.042.960388
GTCTCGC8400.042.908961
TCGCTCT10150.037.5952344
CTGTCGC11100.035.674899
TCTCGCT11400.032.876932
GTCTTGC19350.031.6661451
GTATTAA4800.030.0362721
GTACTAT4000.028.834821
CGCTCTG11050.028.6690565
CTCGCTC11950.027.7342743
GTCTTAC8150.027.4196171
GTGTATA9750.027.3561121
TTGCTCT23050.026.8627514
GTCTCAC21800.025.4619391
GGAGTGC46350.025.1644955
GGGTTTC32600.024.544981
GTTCTAC6200.024.4165821
GAGTGCA50150.024.4062446
GTCCTAT5050.024.2669281
GTATAAT5950.024.2309421
Produced by FastQC (version 0.11.5)