FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345210

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345210
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2358741
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9800.040.410641
TCGCTCT9950.039.7997444
TCTGTCG10300.038.446518
CGCTCTG11500.036.313735
CTCGCTC11450.033.328143
TAATCCG1301.7358032E-533.2313965
GTATTAA4350.033.1054761
GTCTTGC17800.032.3615341
TCTCGCT13050.030.8971332
CGGTTAC1358.416492E-426.6682991
CTGTCGC15700.026.5986659
GGGTAAT5950.024.2031631
GTTAGCC11050.024.1090533
CTCGATC11750.023.8978188
GTACTAG5150.023.7684461
GTCTTAC7600.023.6856631
GTCTCAC23650.023.4432961
GGAGTGC45800.023.4240235
GTCTCGA20300.023.409316
GCGTTAC1550.001882859423.227231
Produced by FastQC (version 0.11.5)