FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871310
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6700.046.207918
TCGCTCT7350.042.1214944
GTCTCGC7800.037.8938981
GTCTTGC13750.036.7007831
TTATACT3950.032.8093874
TCTCGCT8550.032.0275152
GTGTTAC5250.030.209491
GTATTAG4150.029.5311811
CTGTCGC10600.028.5276559
CTCGCTC9650.028.366853
GTCTCAC18050.028.3570581
GTCTAGT4600.028.2094551
GTTAGCC8750.027.9914673
GTATTAT4400.027.8532731
TTGCTCT17600.027.408474
ACCGTGT5000.027.3593798
CGCTCTG10050.027.2232635
GTCTCGA17000.027.1052686
GTATAAC2405.574475E-827.034061
GTCTTAC5950.026.6554341
Produced by FastQC (version 0.11.5)