FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1871310
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8250.044.508978
TCGCTCT8350.042.2525064
GTCTCGC7750.040.879491
GTCTTGC14050.037.4111441
CGCTCTG10900.033.0283165
CTCGCTC10150.032.6313253
TTGCTCT17900.031.7772984
TCTCGCT10000.030.2407252
GTATTGC4050.028.4458851
TATACTT3301.8189894E-1228.3643155
GTATTAT5150.027.9625821
GTCTAAG4450.027.5070111
GGGTACA7100.027.3816681
GTACCGC1607.158293E-527.001371
CTTGCTC17400.026.8971963
GTCTCAC16800.026.5727731
GTATTGG3555.456968E-1226.3675331
TCACTCT17950.026.0730464
CTGTCGC13850.025.4728559
GTACAGA8550.025.2644391
Produced by FastQC (version 0.11.5)