FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555315
Sequences flagged as poor quality0
Sequence length150
%GC50

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9950.047.0340238
TCGCTCT9250.038.139614
GTCTTGC18150.037.335831
CTGTCGC14600.035.012849
TCTCGCT11150.032.319722
CTCGCTC11650.032.1579483
GTCTCGC11250.032.0399671
GCGTTAA1204.196963E-430.037471
TTGCTCT22700.029.1799034
GTCTAGC8350.028.490631
GTCTTAA7100.028.429831
GTATTTA6200.027.9057791
GTGTTAG14400.027.5343461
GTGTAAC6350.027.2465881
GTTTCAC30800.027.1342723
CGCTCTG12250.027.0360725
GGGTTTC34150.026.5983331
TAATACT5700.026.5256674
GTCTTAC8450.026.4471931
GTATAAG6550.026.414631
Produced by FastQC (version 0.11.5)