FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345214

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345214
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2555315
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC18350.043.1647641
GTCTCGC8450.040.90321
TCTGTCG11850.038.885888
CGCTCTG11600.031.6562485
TCGCTCT11150.031.6423324
GTATTAA5150.030.7601321
TTGCTCT25750.030.7583274
CTGTCGC17500.029.2112799
CTTGCTC25500.028.8009783
GTACTAG4300.028.4677541
GTACTAA3650.027.6191
GTCTTAC7400.027.2457681
CTCGCTC13600.027.0009163
TCTCGCT12950.026.6881642
GTATAGA7300.026.6326071
GTGTTAG14750.026.3617631
GTCTCAC21900.026.3038061
GGGTTTC31550.026.2464891
TAGTCCG1400.001040878425.715165
GTATTAT6450.025.67681
Produced by FastQC (version 0.11.5)