FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1841358
Sequences flagged as poor quality0
Sequence length150
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7100.047.660868
TCGCTCT9000.038.39914
GTCTCGC8550.037.938151
CTGTCGC9900.037.0900429
GTCTTGC14000.033.4668661
TTCACCG4650.032.515375
CTCGCTC10000.032.421253
GCGTTAA900.003733111532.036661
TAACCGT900.003754528231.9992524
TCTCGCT9700.031.947142
TACACCG1153.2945807E-431.3036175
ACCGTGT5300.031.2445538
CGCTCTG10450.031.004065
CTCGATC8150.030.9195238
TCTCGAT7750.030.6573477
GGGTTTC23150.030.2030281
GTTTCAC22800.030.0196763
CCGATCG1204.2278878E-429.99939
TCGATCT8200.029.852969
GTATTAT5050.028.5475181
Produced by FastQC (version 0.11.5)