FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345218

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345218
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2357960
Sequences flagged as poor quality0
Sequence length150
%GC51

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9750.038.4030881
TCTGTCG11200.037.285558
GTCTTGC16750.035.2505951
GTCTAAG5850.032.0025751
GTCTTAC8000.031.5025331
TCGCTCT11750.030.6394624
CTCGCTC13200.029.4556663
GTATTAT7000.028.8023151
TCTCGCT12850.028.0166282
GTCTCAC25100.027.5400621
GTGTTAG11600.027.3125421
GGGTTTC27600.027.1326161
TTGCTCT22350.026.0949524
CGCTCTG13300.025.9859495
GTGTAAC5850.025.8482321
TCACTCT25800.025.3963134
GTTAGCC9100.025.3196433
GTATTAC5000.024.4819681
GTATTAG5300.024.4547941
CCGTGTT6200.024.386999
Produced by FastQC (version 0.11.5)