FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2195557
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTTGC17450.035.1195641
GTACCGC1402.8544373E-530.8993191
TCTGTCG8950.030.5690888
GTGTAAC6000.028.8393611
GTATTAA7600.027.5112341
TCGCTCT8350.026.7298894
TACCGTT1358.4205106E-426.6660187
GTGTTAG12200.026.5936741
GTATTAT5700.026.562571
GTGTTAC8150.026.5392911
GTCTCGC7100.026.4022331
TTCACCG5250.026.0565075
GTATTAG7550.025.7835351
TAATACT6050.024.9911274
TCGACCG1450.001277592724.8269829
CTGTCGC11700.024.6147869
GTCTCAC20250.024.5668621
GTATAAA7850.023.8797261
GTCTTAC6700.023.6741031
GTGTATA11300.023.6074431
Produced by FastQC (version 0.11.5)