FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3980737
Sequences flagged as poor quality0
Sequence length150
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCAC25100.023.9301511
GTCTTGC29750.022.1358131
GGAGTGC54950.021.8816595
GTCTAGC10000.021.7101211
TCTGTCG15600.021.6941648
GAGTGCA59000.021.5999496
CTGGAGT66000.021.0537033
GTGTTAG13950.020.7504161
GTGTAAA14050.020.6027261
TTGCTCT29500.020.5021634
GTGTAAC7350.019.6917231
GTTAGCC12800.019.1242353
GTCTAAG8000.018.9963571
TGGAGTG69700.018.8010034
GGCTAGA14400.018.5943181
GTTCAGA15600.018.555661
TTACACT10650.018.2539374
GGCTGGA93200.018.2470651
AGTGCAG59900.017.7896087
TGTTAGC14700.017.6354922
Produced by FastQC (version 0.11.5)