FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2661628
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9900.043.638378
GTCTTGC19800.034.713351
CTGTCGC13800.033.9139639
GTCTTAC9250.032.8507771
GTCTCGC11600.030.5616071
GGGTAAC7950.028.2119241
TCGCTCT12350.027.4041274
GTCTCAC28050.027.3407941
GCGGTTA1607.164219E-526.9987036
TCTCGCT13300.025.9925062
TACCCCG2851.067383E-825.2619445
TTGCTCT27650.025.2616464
TTACTCT13650.024.794214
TCTCGAT9750.024.3680617
GTCTCGA19950.024.179296
GTATTAC6950.023.9431421
CTCGATC9400.023.7457718
GATCGTT3052.1926098E-823.6071999
TCGATCT9600.023.2506319
GTATAAG7800.023.189081
Produced by FastQC (version 0.11.5)