FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005345228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005345228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2218540
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8550.047.136758
TAATACT6150.033.935234
GTATAGG5950.032.1532021
GTATTAG7600.031.9498041
GTCTTGC16450.031.311221
CTGTCGC12500.030.5198149
GTGTTAG14300.030.3587471
TCGCTCT9650.029.8305054
GTCTCGC9300.029.2743071
GTCTTAC7200.027.5930121
AACCGCG1856.1634437E-627.2310267
GGGTTTC30900.026.670271
GTCTCAC21300.026.2543831
TCTCGCT11100.025.2984962
GGGTTAT7050.025.0489771
CGCTCTG10350.025.0316835
GTACTAG3551.2914825E-1024.8725741
GTACAAG6850.024.7061541
TTGCTCT23350.024.6564774
GTTTCAC28850.024.446023
Produced by FastQC (version 0.11.5)